New analytical tool reveals massive DNA damage caused by CRISPR/Cas9 gene editing
(www.gmwatch.org)https://www.gmwatch.org/en/news/latest-news/19732-new-analytical-tool-reveals-massive-dna-damage-caused-by-crispr-cas9-gene-editing"New research from Chinese scientists shows that CRISPR/Cas9 gene editing causes massive damage to the genome, much of which would have been missed by the analytical tools used so far.
In a previous study, the same authors described a novel DNA sequencing procedure, which they called a “primer-extension-mediated sequencing assay” or PEM-seq. By applying this procedure, they found that DNA double-strand breaks (DSBs) brought about by the CRISPR/Cas9 gene-editing tool could lead to unintended chromosomal translocations and large deletions."
